4D60
Structure of a dimeric Plasmodium falciparum profilin mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.070, 246.460, 256.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.991 - 3.300 |
| R-factor | 0.241 |
| Rwork | 0.240 |
| R-free | 0.26320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jkf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.129 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.390 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.140 | |
| Number of reflections | 154448 | |
| <I/σ(I)> | 8.1 | 0.7 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 3.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1.8 M AMMONIUM SULFATE, 0.1 M 2-(N- MORPHOLINO)ETHANESULFONIC ACID (PH 6) |
