4D48
Crystal Structure of glucose-1-phosphate uridylyltransferase GalU from Erwinia amylovora.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 62 |
| Unit cell lengths | 80.673, 80.673, 169.182 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.660 - 2.460 |
| R-factor | 0.24506 |
| Rwork | 0.244 |
| R-free | 0.27311 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e3d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.514 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.660 | 2.560 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.100 | 0.430 |
| Number of reflections | 12434 | |
| <I/σ(I)> | 12.1 | 3.4 |
| Completeness [%] | 99.4 | 94.9 |
| Redundancy | 8.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 2M AMMONIUM SULFATE, 0.1 M TRIS PH 8.5, 5% ETHYLENE GLYCOL |
