4D3P

crystal structure of point mutated DUSP19 (C150A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-06
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	47.183, 50.147, 57.687
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.270
R-factor	0.15624
Rwork	0.155
R-free	0.17762
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s4e
RMSD bond length	0.024
RMSD bond angle	2.277
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.200
High resolution limit [Å]	1.180	1.180
Rmerge	0.080	0.360
Number of reflections	45782
<I/σ(I)>	6.8	5.6
Completeness [%]	100.0	100
Redundancy	7.1	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				1.8M AMMONIUM SULFATE