4D2M

Vaccinia Virus F1L bound to Bim BH3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 41 21 2
Unit cell lengths	56.503, 56.503, 232.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.788 - 2.100
R-factor	0.2168
Rwork	0.215
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vty
RMSD bond length	0.003
RMSD bond angle	0.626
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.540	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.060	0.480
Number of reflections	23011
<I/σ(I)>	23.2	5.3
Completeness [%]	99.9	100
Redundancy	11.7	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.2		0.2M KCL, 15 % PEG400, 1.44 % MPD AND 0.1M AMMONIUM CITRATE PH 6.2.