4D2L
Vaccinia Virus F1L bound to Bak BH3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 116.691, 116.691, 103.639 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.745 - 2.903 |
| R-factor | 0.2025 |
| Rwork | 0.201 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vty |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.740 | |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.050 | 0.720 |
| Number of reflections | 9630 | |
| <I/σ(I)> | 39.6 | 4.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.18 M AMMONIUM SULFATE AND 2.25 M LICL., pH 7.5 |
