4D2G
Crystal structure of human PCNA in complex with p15 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.053, 41.228, 141.121 |
| Unit cell angles | 90.00, 105.39, 90.00 |
Refinement procedure
| Resolution | 44.350 - 2.650 |
| R-factor | 0.1744 |
| Rwork | 0.170 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vym |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.786 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.350 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.090 | 0.510 |
| Number of reflections | 24870 | |
| <I/σ(I)> | 9.3 | 2 |
| Completeness [%] | 98.8 | 89.1 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 40% PEG 300 IN 0.1 MM PHOSPHATE-CITRATE PH 4.2 |
