4D28
Crystal structure of the kinase domain of CIPK24/SOS2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.111, 71.353, 77.834 |
| Unit cell angles | 104.85, 100.32, 118.96 |
Refinement procedure
| Resolution | 70.230 - 3.300 |
| R-factor | 0.2712 |
| Rwork | 0.271 |
| R-free | 0.28312 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4czt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.126 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.200 | 3.600 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.270 | 1.210 |
| Number of reflections | 15242 | |
| <I/σ(I)> | 5.3 | 1.4 |
| Completeness [%] | 91.0 | 92 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20 MM TRIS PH 7.0 AND 22% PEG 4K. |
