4D1W
A H224Y mutant for VIM-7 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 43 |
| Unit cell lengths | 70.225, 70.225, 47.027 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.400 |
| R-factor | 0.11969 |
| Rwork | 0.118 |
| R-free | 0.15636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y8b |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.520 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.070 | 0.160 |
| Number of reflections | 43649 | |
| <I/σ(I)> | 9.1 | 0 |
| Completeness [%] | 96.6 | 86.8 |
| Redundancy | 3.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
