4D1V
A F218Y mutant of VIM-7 from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 43 |
Unit cell lengths | 69.947, 69.947, 46.936 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.730 - 1.700 |
R-factor | 0.1613 |
Rwork | 0.159 |
R-free | 0.19650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y8b |
RMSD bond length | 0.009 |
RMSD bond angle | 1.158 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.060 | 0.560 |
Number of reflections | 25160 | |
<I/σ(I)> | 16.3 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 25% PEG 3350, 0.1 M BISTRIS PH 5.5, 0.2 M NACL |