4D1Q
Hermes transposase bound to its terminal inverted repeat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Collection date | 2011-02-17 |
| Detector | RIGAKU IMAGING PLATE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 265.150, 265.150, 157.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.400 |
| R-factor | 0.2118 |
| Rwork | 0.212 |
| R-free | 0.25430 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.000 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.300 |
| Rmerge | 0.180 |
| Number of reflections | 94693 |
| <I/σ(I)> | 12.54 |
| Completeness [%] | 98.7 |
| Redundancy | 13.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 3 UL OF THE HERMES 79-612/L16-1T COMPLEX AT 8 MG/ML PROTEIN WAS MIXED WITH 1 UL WELL SOLUTION CONSISTING OF 0.18 M KCL, 5 MM DTT, 0.8 M K, NA TARTRATE, 0.1 M MES PH 6.5, AND 5% (V/V) T-BUTANOL. |
