4D1L

Tetramerization domain of zebrafish p53 (crystal form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 2 2 21
Unit cell lengths	87.853, 120.611, 61.029
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.500 - 1.970
R-factor	0.21876
Rwork	0.217
R-free	0.24698
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c26
RMSD bond length	0.011
RMSD bond angle	1.342
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.500	2.080
High resolution limit [Å]	1.970	1.970
Rmerge	0.050	0.650
Number of reflections	23226
<I/σ(I)>	18.1	2.8
Completeness [%]	99.6	99.3
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	SITTING DROP VAPOR DIFFUSION AT 20 DEGREE C; PROTEIN SOLUTION: 12 MG/ML IN 20 MM TRIS PH 7.5, 50 MM NACL, 5 MM DTT; CRYSTALLIZATION BUFFER: 50 MM ZINC ACETATE AND 20% (W/V) POLYETHYLENE GLYCOL 3,350.