4D1I
The structure of the GH35 beta-galactosidase Bgl35A from Cellvibrio japonicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 98.911, 115.783, 116.036 |
| Unit cell angles | 90.21, 90.25, 90.38 |
Refinement procedure
| Resolution | 116.030 - 1.800 |
| R-factor | 0.18617 |
| Rwork | 0.185 |
| R-free | 0.21153 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u7v |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.476 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.630 |
| Number of reflections | 459893 | |
| <I/σ(I)> | 8.7 | 1.1 |
| Completeness [%] | 96.4 | 95.1 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.8 M SODIUM ACETATE PH 7.0 |
