4D18
Crystal structure of the COP9 signalosome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 |
| Unit cell lengths | 147.680, 147.680, 317.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.980 - 4.080 |
| R-factor | 0.237 |
| Rwork | 0.236 |
| R-free | 0.25340 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.890 |
| Data reduction software | XDS |
| Data scaling software | XDS (AIMLESS) |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.000 | 4.090 |
| High resolution limit [Å] | 4.080 | 4.080 |
| Rmerge | 0.160 | |
| Number of reflections | 61692 | |
| <I/σ(I)> | 6.7 | 0.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.4 | CRYSTAL GROWN BY VAPOR DIFFUSION BY MIXING 9.3 MG/ML PROTEIN IN 50 MM HEPES PH 7.4, 200 MM NACL, 2 MM EQUALLY WITH 12% PEG 6000, 100 MM TRISODIUM CITRATE PH 5.4, 0.1 M LI2SO4. CRYSTAL DEHYDRATED OVERNIGHT WITH 20% PEG 6000 IN WELL |
