4D12
Crystal Structure of Cofactor-free Urate Oxidase Anaerobically Complexed with Uric Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | AGILENT NOVA |
| Temperature [K] | 100 |
| Collection date | 2013-11-11 |
| Detector | AGILENT |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.918, 94.870, 104.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.140 - 1.400 |
| R-factor | 0.11904 |
| Rwork | 0.118 |
| R-free | 0.14189 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.274 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.530 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 0.330 |
| Number of reflections | 76756 | |
| <I/σ(I)> | 14.9 | 2.4 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20 MG/ML UOX IN 50 MM TRIS-HCL PH 8.0 SATURATED WITH URIC ACID 8% PEG 8000, 50 MM TRIS-HCL PH 8.0 CRYSTALS GROWN ANAEROBICALLY |
