4D0M
Phosphatidylinositol 4-kinase III beta in a complex with Rab11a-GTP- gamma-S and the Rab-binding domain of FIP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 199.504, 134.465, 294.328 |
| Unit cell angles | 90.00, 90.33, 90.00 |
Refinement procedure
| Resolution | 294.320 - 6.000 |
| R-factor | 0.25888 |
| Rwork | 0.253 |
| R-free | 0.35946 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d0l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.366 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.880 | 6.270 |
| High resolution limit [Å] | 6.000 | 6.000 |
| Rmerge | 0.170 | 1.030 |
| Number of reflections | 37822 | |
| <I/σ(I)> | 17.8 | 2.2 |
| Completeness [%] | 95.9 | 97 |
| Redundancy | 5.6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 16% PEG 6K, 0.01 M NA CITRATE |
