4D0G
Structure of Rab14 in complex with Rab-Coupling Protein (RCP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.870, 148.228, 38.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.500 - 2.500 |
| R-factor | 0.23113 |
| Rwork | 0.224 |
| R-free | 0.29438 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4drz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.302 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.800 |
| Number of reflections | 9973 | |
| <I/σ(I)> | 8.5 | 1.72 |
| Completeness [%] | 96.5 | 70.1 |
| Redundancy | 8.9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.75 | 14% PEG 8000, 0.1 M HEPES PH 6.75 |
