4D0D
COMPLEX OF A B2 CHICKEN MHC CLASS I MOLECULE AND A 8MER CHICKEN PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Collection date | 2011-12-10 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.080, 92.540, 223.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.950 - 3.130 |
| R-factor | 0.2928 |
| Rwork | 0.292 |
| R-free | 0.29860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2YF6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.820 |
| Data reduction software | XDS (THROUGH XIA2) |
| Data scaling software | Aimless (THROUGH XIA2) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.080 | 3.210 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Rmerge | 0.180 | 0.690 |
| Number of reflections | 32916 | |
| <I/σ(I)> | 8.8 | 2.1 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1M MMT BUFFER, PH 7.0, 25% W/V PEG 1500 |
