4D07

DYNLL2 dynein light chain binds to an extended, unstructured linear motif of myosin 5a tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-09
Detector	ADSC QUANTUM 315r
Spacegroup name	P 61 2 2
Unit cell lengths	45.160, 45.160, 204.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	204.010 - 1.850
R-factor	0.19492
Rwork	0.193
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cmi
RMSD bond length	0.007
RMSD bond angle	1.113
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.760	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.100	0.700
Number of reflections	11478
<I/σ(I)>	30.18	6.09
Completeness [%]	99.8	100
Redundancy	22	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				2.5UL PROTEIN PLUS 1UL RESERVOIR SOLUTION OF 1.8 M (NH4)2SO4, 0.02 M COCL2, 0.1 M MES PH 6.5