4D01
Crystal Structure of the Extracellular Domain of the Human Alpha9 Nicotinic Acetylcholine Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-10 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 49.520, 64.269, 80.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.126 - 1.795 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qc1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.459 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 42.000 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.080 | 1.400 |
| Number of reflections | 24313 | |
| <I/σ(I)> | 15.9 | 1.2 |
| Completeness [%] | 99.3 | 95.4 |
| Redundancy | 12.5 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM SPG BUFFER PH 6.5, 25% PEG 3350 |
