4CZK

C. crescentus MreB, single filament, AMPPNP, MP265 inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	51.754, 73.264, 84.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.198 - 2.602
R-factor	0.1981
Rwork	0.196
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jce
RMSD bond length	0.002
RMSD bond angle	0.617
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.130	0.780
Number of reflections	10352
<I/σ(I)>	14.1	3.2
Completeness [%]	99.9	99.9
Redundancy	11.1	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1