4CZG
C. crescentus MreB, single filament, ADP, A22 inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.674, 73.678, 54.233 |
| Unit cell angles | 90.00, 102.39, 90.00 |
Refinement procedure
| Resolution | 20.397 - 1.500 |
| R-factor | 0.1666 |
| Rwork | 0.164 |
| R-free | 0.21770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jce |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.385 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.690 |
| Number of reflections | 52173 | |
| <I/σ(I)> | 19.8 | 1.1 |
| Completeness [%] | 87.8 | 77 |
| Redundancy | 6.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
