4CZ9

Structure of the sodium proton antiporter PaNhaP from Pyrococcus abyssii at pH 4.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-03
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 64
Unit cell lengths	109.566, 109.566, 209.626
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.500
Rwork	0.242
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cz8
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.720
High resolution limit [Å]	3.500	3.500
Rmerge	0.020	0.490
Number of reflections	33232
<I/σ(I)>	19.9	1.9
Completeness [%]	100.0	100
Redundancy	9.2	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4		pH 4.0