4CZ2
Complex of human VARP-ANKRD1 with Rab32-GppCp. Selenomet derivative.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.050, 122.490, 131.580 |
| Unit cell angles | 90.00, 113.25, 90.00 |
Refinement procedure
| Resolution | 120.890 - 2.970 |
| R-factor | 0.1968 |
| Rwork | 0.195 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cym |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.580 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.050 | 3.120 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.220 | 0.990 |
| Number of reflections | 33062 | |
| <I/σ(I)> | 7.8 | 1.9 |
| Completeness [%] | 97.3 | 98.3 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
