4CXV
Structure of bifunctional endonuclease (AtBFN2) in complex with phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-26 |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.524, 52.870, 60.841 |
| Unit cell angles | 71.34, 78.63, 76.62 |
Refinement procedure
| Resolution | 25.430 - 2.000 |
| R-factor | 0.20423 |
| Rwork | 0.202 |
| R-free | 0.24942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cwm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.563 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.260 |
| Number of reflections | 34563 | |
| <I/σ(I)> | 9.4 | 3.3 |
| Completeness [%] | 96.7 | 95.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES, PH 7.5 0.2 M NA2HPO4 30% (W/V) PEG3350 |
