4CX9
The 5-coordinate proximal NO complex of cytochrome c prime from Shewanella frigidimarina
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.300, 58.279, 76.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.280 - 1.430 |
| R-factor | 0.16232 |
| Rwork | 0.161 |
| R-free | 0.18631 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yli |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.131 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.100 | 1.510 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.080 | 0.770 |
| Number of reflections | 46165 | |
| <I/σ(I)> | 12.6 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.2M AM SULFATE, 0.1M HEPES PH7 |
