4CX8
Monomeric pseudorabies virus protease pUL26N at 2.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.437, 101.337, 130.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.940 - 2.530 |
| R-factor | 0.20078 |
| Rwork | 0.198 |
| R-free | 0.25215 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DIMERIC PRV PROTEASE YET TO BE DEPOSITED |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.788 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.680 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.080 | 0.660 |
| Number of reflections | 18230 | |
| <I/σ(I)> | 14.29 | 2.06 |
| Completeness [%] | 97.8 | 93.6 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M TRIS PH 8, 14% PEG 20,000 |
