4CWM
High-glycosylation crystal structure of the bifunctional endonuclease (AtBFN2) from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-10 |
| Detector | ADSC QUANTUM |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.375, 52.706, 61.105 |
| Unit cell angles | 71.34, 78.57, 76.74 |
Refinement procedure
| Resolution | 23.280 - 2.090 |
| R-factor | 0.20108 |
| Rwork | 0.197 |
| R-free | 0.26802 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w52 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.564 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.280 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.010 | 0.450 |
| Number of reflections | 28226 | |
| <I/σ(I)> | 14.7 | 3.7 |
| Completeness [%] | 97.3 | 88 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5 0.2 M SODIUM ACETATE 30% W/V PEG4000 |
