4CWE
Structural studies of rolling circle replication initiation protein from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-07 |
| Detector | ADSC Q4 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 168.242, 168.242, 168.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.660 - 3.000 |
| R-factor | 0.24364 |
| Rwork | 0.241 |
| R-free | 0.29711 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cij |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.578 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.560 |
| Number of reflections | 15902 | |
| <I/σ(I)> | 23.7 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M SODIUM CITRATE PH 5.5, 2.5 M 1,6-HEXANEDIOL |
