4CVL
PaMurF in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.580, 87.380, 91.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.200 - 2.980 |
| R-factor | 0.21766 |
| Rwork | 0.214 |
| R-free | 0.29498 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.525 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.200 | 3.060 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.190 | 1.020 |
| Number of reflections | 10089 | |
| <I/σ(I)> | 12.9 | 3.9 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 12.7 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M CACL2, 0.1M TRIS (PH 8.5), 20 % PEG 6K |
