4CVH
Crystal structure of human isoprenoid synthase domain-containing protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-17 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.360, 116.800, 111.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.640 - 2.390 |
| R-factor | 0.1912 |
| Rwork | 0.189 |
| R-free | 0.24369 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.149 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Rosetta |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.640 | 2.500 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.070 | 0.430 |
| Number of reflections | 20791 | |
| <I/σ(I)> | 8.6 | 2.5 |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M BIS-TRIS PH 6.5, 25%(W/V) PEG 3350 |
