4CVB
Crystal structure of quinone-dependent alcohol dehydrogenase from Pseudogluconobacter saccharoketogenenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-02 |
| Detector | MACSCIENCE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.950, 87.190, 57.100 |
| Unit cell angles | 90.00, 90.16, 90.00 |
Refinement procedure
| Resolution | 27.950 - 1.720 |
| R-factor | 0.12872 |
| Rwork | 0.128 |
| R-free | 0.14858 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1KB0 AND 1YIQ |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.357 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 1.840 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.080 | 0.230 |
| Number of reflections | 59031 | |
| <I/σ(I)> | 11.4 | 4.3 |
| Completeness [%] | 88.9 | 31.3 |
| Redundancy | 3.3 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 18-21% PEG550 MME, 20 MM ZN(AC)2, 100 MM PCB BUFFER PH 6-7 |
