4CV4
PIH N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-01 |
| Detector | SATURN |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.860, 50.140, 35.180 |
| Unit cell angles | 90.00, 103.21, 90.00 |
Refinement procedure
| Resolution | 34.249 - 1.902 |
| R-factor | 0.1207 |
| Rwork | 0.119 |
| R-free | 0.15850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ckt |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.255 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.250 | 2.160 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.170 |
| Number of reflections | 8928 | |
| <I/σ(I)> | 22.7 | 14.9 |
| Completeness [%] | 98.1 | 96.4 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.02M MAGNESIUM CHLORIDE HEXAHYDRATE, 0.002M COBALT (2) CHLORIDE, 0.05M HEPES PH 7.5, 2M AMMONIUM SULFATE, 0.001M SPERMINE |
