4CUD
Human Notch1 EGF domains 11-13 mutant fucosylated at T466
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 28.290, 28.290, 282.732 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.140 - 1.850 |
| R-factor | 0.22572 |
| Rwork | 0.224 |
| R-free | 0.25992 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vj3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.112 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.140 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.070 | 0.420 |
| Number of reflections | 10837 | |
| <I/σ(I)> | 0 | 3 |
| Completeness [%] | 93.5 | 95.3 |
| Redundancy | 3.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6.0 |
