4CUC
Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 117.340, 117.340, 219.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 117.340 - 2.200 |
| R-factor | 0.15532 |
| Rwork | 0.153 |
| R-free | 0.19127 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.452 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.490 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.200 | 0.640 |
| Number of reflections | 78624 | |
| <I/σ(I)> | 10.1 | 5.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
