4CSH
Native structure of the lytic CHAPK domain of the endolysin LysK from Staphylococcus aureus bacteriophage K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.220, 61.470, 73.130 |
| Unit cell angles | 91.49, 98.62, 89.99 |
Refinement procedure
| Resolution | 32.850 - 1.790 |
| R-factor | 0.17598 |
| Rwork | 0.175 |
| R-free | 0.20106 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INTERMEDIATE MODEL BASED ON DERIVATIVE DATA PDB ENTRY 4CT3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.470 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.530 | 1.880 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.090 | 0.250 |
| Number of reflections | 62028 | |
| <I/σ(I)> | 6.3 | 2.9 |
| Completeness [%] | 97.2 | 94.3 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 25 MM TRIS-HCL, 22% (W/V) POLYETHYLENE GLYCOL 8000, 0.1 M 2-(N-MORPHOLINO)ETHANESULFONIC ACID-NAOH PH 6.5 |
