4CSG
Structural insights into Toscana virus RNA encapsidation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 104.484, 104.484, 510.763 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.124 - 3.320 |
| R-factor | 0.241 |
| Rwork | 0.240 |
| R-free | 0.26780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HOMOLOGY MODEL BASED ON 3OUO |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.713 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.410 |
| High resolution limit [Å] | 3.320 | 3.320 |
| Rmerge | 0.010 | 0.510 |
| Number of reflections | 45886 | |
| <I/σ(I)> | 10.7 | 2.4 |
| Completeness [%] | 99.1 | 94.5 |
| Redundancy | 4.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 6.5% PEG 3350, 0.1 M SODIUM MALONATE, PH 4.5-5.2 |
