4CSF
Structural insights into Toscana virus RNA encapsidation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 98.777, 127.837, 170.467 |
| Unit cell angles | 82.10, 79.75, 74.49 |
Refinement procedure
| Resolution | 34.467 - 2.598 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.23960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.847 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.400 | 2.610 |
| High resolution limit [Å] | 2.600 | 2.590 |
| Rmerge | 0.090 | 0.555 |
| Number of reflections | 229780 | |
| <I/σ(I)> | 11.2 | 2.1 |
| Completeness [%] | 94.7 | 79.7 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 11% PEG 3350, 0.1 M SODIUM MALONATE, PH 4.5-5.2 |
