4CS7
Crystal structure of the asymmetric human metapneumovirus M2-1 tetramer, form 1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.2828, 1.2802 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.150, 92.670, 82.830 |
Unit cell angles | 90.00, 94.48, 90.00 |
Refinement procedure
Resolution | 33.130 - 2.470 |
R-factor | 0.2348 |
Rwork | 0.233 |
R-free | 0.26150 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.180 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.130 | 2.530 |
High resolution limit [Å] | 2.470 | 2.470 |
Rmerge | 0.080 | 1.340 |
Number of reflections | 27190 | |
<I/σ(I)> | 12.2 | 1.7 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 28 % W/V POLYETHYLENE GLYCOL MONOMETHYL ETHER 2000, 0.100 M BIS-TRIS PH 6.5 |