4CRV

Complex of human CNOT9 and CNOT1 including two tryptophans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2013-08-19
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 1 2 1
Unit cell lengths	154.070, 66.810, 71.990
Unit cell angles	90.00, 100.22, 90.00

Refinement procedure

Resolution	61.138 - 2.050
R-factor	0.178
Rwork	0.176
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cru
RMSD bond length	0.006
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.100	2.100
High resolution limit [Å]	2.050	2.050
Number of reflections	45149
<I/σ(I)>	14.9	1.8
Completeness [%]	99.5	99
Redundancy	6.8	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1M MES (PH=6.0), 11% PEG6000, 0.05M MG-CHLORIDE, 0.04M L-TRYPTOPHAN