4CRU

Complex of human CNOT9 and CNOT1 including one tryptophan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2013-03-20
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 1 2 1
Unit cell lengths	154.800, 67.240, 72.300
Unit cell angles	90.00, 99.64, 90.00

Refinement procedure

Resolution	76.307 - 1.650
R-factor	0.1606
Rwork	0.160
R-free	0.17880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2FV2 CHAIN A
RMSD bond length	0.011
RMSD bond angle	1.266
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.300	1.690
High resolution limit [Å]	1.650	1.650
Number of reflections	87658
<I/σ(I)>	19.8	1.8
Completeness [%]	99.5	99
Redundancy	3.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1M MES (PH=6.0), 8% PEG6000, 0.08M MG-CHLORIDE