4CRS
Human Protein Kinase N2 (PKN2, PRKCL2) in complex with ATPgammaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.639, 68.639, 185.473 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.820 - 2.750 |
| R-factor | 0.21251 |
| Rwork | 0.210 |
| R-free | 0.26201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o6l |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.188 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.820 | 2.880 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.120 | 1.200 |
| Number of reflections | 13681 | |
| <I/σ(I)> | 6.2 | 1.3 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 2.3M AMMONIUM SULPHATE, 0.1M CITRATE PH 5.8, 0.2M SODIUM/POTASSIUM TARTRATE |
