4CQX
H5 (tyTy) Del133/Ile155Thr Mutant Haemagglutinin in Complex with Human Receptor Analogue 6'SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.824, 117.104, 101.275 |
| Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
| Resolution | 101.170 - 2.300 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.22612 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bgz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.238 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.730 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.650 |
| Number of reflections | 90479 | |
| <I/σ(I)> | 9.9 | 2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BIS-TRIS PROPANE PH 7.5, 0.05 - 0.15 M K/NAPO4 (PH 7.0), 15-18% PEG 3350 |
