4CQV
Crystal structure of H5 (tyTy) Del133/Ile155Thr Mutant Haemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.407, 117.251, 101.003 |
| Unit cell angles | 90.00, 92.52, 90.00 |
Refinement procedure
| Resolution | 70.130 - 2.860 |
| R-factor | 0.19778 |
| Rwork | 0.195 |
| R-free | 0.24444 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bgz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.170 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.030 | 3.010 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 46268 | |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 98.3 | 99 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BIS-TRIS PROPANE PH 7.5, 0.05 - 0.15 M K/NAPO4 (PH 7.0), 15-18% PEG 3350 |
