4CQO
Structure of the human CNOT1 superfamily homology domain in complex with a Nanos1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.530, 167.330, 112.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.452 - 2.800 |
| R-factor | 0.2227 |
| Rwork | 0.222 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c0d CHAIN A |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.628 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.3_1479)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.520 | 2.860 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 45219 | |
| <I/σ(I)> | 11.8 | 2.4 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 10.7 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.7 M SUCCINIC ACID SODIUM SALT, PH 7.0 |
