4CPO

Structure of the Neuraminidase from the B/Lyon/CHU/15.216/2011 virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 2M
Spacegroup name	P 32 2 1
Unit cell lengths	159.490, 159.490, 89.547
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.041 - 2.200
R-factor	0.1546
Rwork	0.153
R-free	0.19040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cpl
RMSD bond length	0.007
RMSD bond angle	1.149
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.040	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.140	0.640
Number of reflections	64990
<I/σ(I)>	7.5	2.3
Completeness [%]	97.7	98.5
Redundancy	3	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				25% PEG 1500, 0.1M SUCCINIC ACID PH 9.0