4CPE
Wild-type streptavidin in complex with love-hate ligand 1 (LH1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.590, 93.930, 104.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.368 - 1.060 |
| R-factor | 0.1332 |
| Rwork | 0.132 |
| R-free | 0.15350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ry1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.419 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.370 | 1.100 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Rmerge | 0.050 | 0.460 |
| Number of reflections | 96520 | |
| <I/σ(I)> | 18.14 | 2.46 |
| Completeness [%] | 93.1 | 55 |
| Redundancy | 5.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 75% SATURATED AMMONIUM SULPHATE, 25% 1M SODIUM ACETATE PH4.5. SITTING DROP |
