4CP3
The structure of BCL6 BTB (POZ) domain in complex with the ansamycin antibiotic rifabutin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-01 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.172, 54.828, 58.159 |
| Unit cell angles | 90.00, 95.21, 90.00 |
Refinement procedure
| Resolution | 57.920 - 2.300 |
| R-factor | 0.20193 |
| Rwork | 0.199 |
| R-free | 0.26875 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r2b |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.875 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.820 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.108 | 0.518 |
| Number of reflections | 9168 | |
| <I/σ(I)> | 9.8 | 4.1 |
| Completeness [%] | 97.0 | 97 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 20% PEG 6000, 100MM SODIUM CITRATE, PH 5. |
