4COI
Crystal structure of the anaerobic ribonucleotide reductase from Thermotoga maritima with glycerol in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-26 |
| Detector | MARRESEARCH MARMOSAIC 165 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.890, 98.820, 86.980 |
| Unit cell angles | 90.00, 112.42, 90.00 |
Refinement procedure
| Resolution | 24.303 - 1.940 |
| R-factor | 0.1823 |
| Rwork | 0.181 |
| R-free | 0.21680 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.995 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.300 | 2.060 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.080 | 0.670 |
| Number of reflections | 87892 | |
| <I/σ(I)> | 11.3 | 2.1 |
| Completeness [%] | 97.1 | 91.9 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | 24 MG/ML, 8% (W/V) PEG3000, 0.1 M CITRATE BUFFER PH 5.3, 5 MM DTT |
