4COF
Crystal structure of a human gamma-aminobutyric acid receptor, the GABA(A)R-beta3 homopentamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.100, 108.900, 207.440 |
| Unit cell angles | 90.00, 107.43, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.970 |
| R-factor | 0.2064 |
| Rwork | 0.205 |
| R-free | 0.22590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rhw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 3.050 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.080 | 0.830 |
| Number of reflections | 76360 | |
| <I/σ(I)> | 13.5 | 2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 11.5% PEG4000 100 MM SODIUM CHLORIDE, 100 MM LITHIUM SULPHATE, 100 MM N-2-ACETAMIDO-IMINODIACETIC ACID, PH 6.5, 2% (W/V) BENZAMIDINE |
