4CO8
Structure of the DNA binding ETS domain of human ETV4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.801, 50.801, 67.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.990 - 1.050 |
| R-factor | 0.11727 |
| Rwork | 0.117 |
| R-free | 0.13201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bnc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.348 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.800 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.040 | 0.670 |
| Number of reflections | 46056 | |
| <I/σ(I)> | 21.1 | 2.1 |
| Completeness [%] | 96.5 | 67.5 |
| Redundancy | 8.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.8 M SODIUM CITRATE TRIBASIC, 0.1 M CACODYLATE PH 6.5. |
